Deep-diving whales can remain active while holding their breath for up to two hours. Figuring out how they do it may help scientists create lifesaving synthetic blood for human trauma patients.

For a new study, researchers compared the muscle protein myoglobin from humans, whales, and other deep-diving mammals. Myoglobin holds oxygen for ready use inside muscle cells. The findings showed that marine mammals have ultra-stable versions of myoglobin that tend to stay folded. That stability is the key for cells to make large amounts of myoglobin, which is why these deep-diving mammals can load their muscle cells with far more myoglobin than humans.

“Whales and other deep-diving marine mammals can pack 10-20 times more myoglobin into their cells than humans can, and that allows them to ‘download’ oxygen directly into their skeletal muscles and stay active even when they are holding their breath,” says John Olson, professor of biochemistry and cell biology at Rice University.

“The reason whale meat is so dark is that it’s filled with myoglobin that is capable of holding oxygen. But when the myoglobin is newly made, it does not yet contain heme. We found that the stability of heme-free myoglobin is the key factor that allows cells to produce high amounts of myoglobin.”

sperm whale

That’s important to Olson because he wants to create a strain of bacteria that can generate massive quantities of another protein that’s closely related to myoglobin. Olson has spent two decades studying hemoglobin, a larger, more complex oxygen-carrying protein in blood. His goal is to create synthetic blood for use in transfusions.

Hospitals and trauma specialists currently rely on donated whole blood, which is often in short supply and has a limited storage life. A crucial part of Olson’s plan is maximizing the amount of hemoglobin that a bacterium can express. “Our results confirm that protein stability is the key,” he says.

“In this study, Premila (Samuel) and George (Phillips) developed an in vitro method for testing myoglobin expression outside of living cells. That allowed us to carefully control all the variables. We found that the amount of fully active myoglobin expressed was directly and strongly dependent on the stability of the protein before it bound the heme group.”

All proteins have a characteristic shape, and the globin family of proteins is shaped around a pocket where heme is stored. The heme pocket opens and closes—much like the pocket of a baseball glove—to trap and release oxygen.

Whole blood is in short supply

The heme-free form of myoglobin is called apoprotein or apomyoglobin, says Samuel, a graduate student in the biosciences department. “The more stable the apoprotein, the more final product we could make. Human apomyoglobin isn’t very stable at all compared to that of the diving mammals, which have versions of the apoglobin that are up to 60 times more stable than ours.”

The stability differences aren’t obvious if one simply compares the overall structures of the myoglobin from each species. Their overall shapes, including the shapes of their heme pockets, are the same. However, thanks to subtle differences in their amino acid sequences, the more stable myoglobins are better able to retain their shapes. This underlying stability only becomes apparent when one studies the heme-free, or “apo” versions of the protein.

For the study, published in the Journal of Biochemistry, Samuel measured stability using chemicals that forced the apoproteins to unfold. By carefully measuring the amount of chemical required, she was able to precisely measure stability.

The work stems from three earlier studies, Samuel says. In 1999, Emily Scott, a graduate student in Olson’s lab, noticed that sperm whale apomyoglobin was much more resistant to chemically induced unfolding than the corresponding human or pig apoproteins. Scott wondered if the resistance to unfolding was a trait of deep-diving whales, so she gathered samples from a variety of mammals and confirmed the idea in 2000.

At the same time, study coauthor Lucian Smith, another of Olson’s graduate students who is now a consultant scientist in the bioengineering department at the University of Washington was examining a catalog of 250 mutant sperm-whale apomyoglobins. He noticed that a certain class of mutations in the heme pocket caused the proteins to become extraordinarily stable even though the mutations damaged their ability to bind heme and oxygen.

Finally, in 2013, Michael Berenbrink of Liverpool University and Kevin Campbell of the University of Manitoba noted that deep-diving mammals expressed large amounts of myoglobin in their muscle tissue.

“At the time, we were in the process of trying to screen large-scale libraries of hemoglobin mutants to try to select for higher stability and expression as part of our work on evaluating blood substitutes,” Olsen says.

Samuel compared the stability and cell-free expression level of myoglobins from humans, pigs, goosebeak whales, gray seals, sperm whales, dwarf sperm whales, and the three mutants, which had low heme affinity but were 50 times more stable than apomyoglobins from the whales. The research confirmed that the stability of apoprotein is directly correlated with expression levels. For example, very little pig and human myoglobin could be made in the cell-free system, which yielded 10- to 20-fold higher amounts of whale and mutant myoglobins.

The results of the cell-free study unequivocally verify the expression-stability correlations that had been anecdotally observed in previous work in both mammalian cells and E. coli, Olson says.

“This work is very important for our projects on synthetic blood substitutes and determining the toxicity of acellular hemoglobin. Premila has laid the groundwork for high-throughput screening of large libraries of hemoglobin variants without the need for purifying milligram quantities of pure protein. This method is a big step forward in our efforts to identify more stable recombinant hemoglobins.”

This text is published here under a Creative Commons License.
Author: Jade Boyd-Rice University
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